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3d coat timelapse10/26/2022 ![]() ![]() In addition, we describe assays to unambiguously image Fcγ receptor- or complement receptor-mediated phagocytosis. Here we describe phagocytic assays, using time-lapse spinning disk confocal microscopy, that allow 3D imaging of single phagocytic events. ![]() More commonly used approaches for the study of phagocytosis, such as end-point assays, miss the opportunity to understand what is happening at the interface of particles and phagocytes. With the availability of high resolution imaging techniques, phagocytosis assays are required that allow real-time 3D (three dimensional) visualization of how specific phagocytic receptors mediate the uptake of individual particles. However, the distinctions between the two modes have become blurred by reports that complement receptor-mediated phagocytosis may induce various membrane protrusions. These two modes of phagocytosis, phagocytic cup formation and sinking in, have become well established in the literature. In contrast, complement opsonized particles appear to sink into the phagocyte following binding to complement receptors. Binding of immunoglobulin G (IgG)-opsonized particles to FcγRs triggers the protrusion of thin membrane extensions, which initially form a so-called phagocytic cup around the particle before it becomes completely enclosed and retracted into the cell. Four decades ago, two distinct mechanisms of phagocytosis, exemplified by Fcγ receptor (FcγR)- and complement receptor (CR)-mediated phagocytosis, were identified using scanning electron microscopy. Although phagocytic receptors, downstream signaling pathways, and effectors, such as Rho GTPases, have been identified, the dynamic cytoskeletal remodeling of specific receptor-mediated phagocytic events remain unclear. After watching this video you will easily be able to master hard surface modeling in 3D Coat for any project you may have.Phagocytosis plays a key role in host defense, as well as in tissue development and maintenance, and involves rapid, receptor-mediated rearrangements of the actin cytoskeleton to capture, envelop and engulf large particles. Leigh?s approach of tool based training will have you using 3D Coat more often in your work flow while also showing how quick 3D Coat?s tools truly can be. #3d coat timelapse professional#This title was created with novice and professional 3D Coat users in mind. If you can start using 3D Coat as a rapid prototype tool, you could turn out projects much faster. In a sculpting package like 3D-Coat this is a very important tool set to learn. This video is really unique as the entire focus is on hard surfaces. #3d coat timelapse how to#Leigh will show you how to not only use the tools but how to quickly produce amazingly accurate results.įrom the cockpit to the landing gear and runs, this is not a beginner project but Leigh’s style and ease of flow will have you sculpting wings and rotors quickly and easily. 3D Coat offers so many powerful tools out of the box, but if you do not understand or know how to properly use them, you will never see the true benefit. You will not find this information for Hard surface Modeling in any other training video on the market currently. ![]() Leigh brings his hallmark style to this ?Official Guide? training video, providing details on using 3D Coat?s toolset. If you are thinking about 3D Coat as your sculpting tool but had reservations as to it?s hard surfacing capabilities? This video will help lay to rest your reservations. This title shows off not only Leigh?s talent, but 3D Coats versatility and amazing tool set for hard surface modeling. #3d coat timelapse series#Bamforth?s second retail video for Pilgway?s 3D Coat and first in a series on advanced projects. ![]()
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